Forskolin

8: Mol Cell Biochem 2002 Nov;240(1-2):131-3

The tyrosine kinase inhibitor genistein increases basal cAMP and potentiates forskolin-induced cAMP accumulation in A549 human airway epithelial cells.

Burvall KM, Palmberg L, Larsson K.

National Institute for Working Life, Stockholm, Sweden. Karin.Burvall@niwl.se

Genistein is often used as an inhibitor of tyrosine kinases. A less studied side effect of genistein is an inhibition of cyclic AMP-phosphodiesterase (cAMP-PDE) activity resulting in increased cAMP accumulation. The effect of genistein on intracellular cAMP-levels, basal and forskolin-induced, was studied in A549 human airway epithelial cells and compared with the unspecific PDE inhibitor, isobutylmethylxanthine (IBMX). It was shown that genistein (50 microM) increased basal cAMP and potentiated forskolin-induced cAMP accumulation to the same extent as IBMX (100 microM). Thus, the use of genistein in studies on signaling transductions may result in erroneous conclusions since increased cAMP may cause or contribute to the observed effects.

PMID: 12487380

[top]


4mg noneto biocompare formulae omd ToxicPlants DictCellBio MolBioDict ( test position ) BioChemical Suppliers

A23187	Actinomycin	Aflatoxin	Anisomycin	Aphidicolin	Ascomycin
Bleomycin	Brefeldin	Cerulenin	Chromomycin	Geldanamycin	Cyclopiazonic acid
Cytochalasin	Forskolin	Fumagillin	Fumonisin	Hypericin	K252a
KT5823	Mitomycin	Nigericin	Ochratoxin	Oligomycin	Mycophenolic acid
Paclitaxel	Patulin	Paxilline	Penitrem	Puromycin	Penicillic acid
Radicicol	Rapamycin	Staurosporine	Sterigmatocystin	Thapsigargin	Tunicamycin
Verruculogen	Wortmannin